he analysis quality of chromosomal DNA extracted from unknown strains in Eclair by enzyme and glass beads. Nanodrop measured the quality of chromosomal DNA at A260/280. Indicative of the contamination of protein were 2.21 and 2.35, respectively. In the purity of the chromosomal DNA is high because the values were higher than 1.8 for the contamination of the phenol, which has a value of A260/A230 were 0.53 and 0.81, respectively. Which high contamination of the phenol because the lower than 1.5 as shown in table 1.