where the final mixture contained1.5 U of Taq DNA polymerase, 10 mM of Tris–HCl pH 9, 50 mM of KCl, 1.5 mM of MgCl, 200 µM of each dNTP,5 pmol of each of the two primers, 100 ng of genomic DNA,and water to a final volume of 25 µL (for IGS) or 50 µL (for 16S rRNA gene).