The ability of the enzyme production by isolates was determined in culture broth. Each strain was cultured in 250 ml Erlenmeyer flask containing 100 ml of basal liquid medium consisting per liter, 1 g; PLA film, 4 g; (NH4)2SO4, 2 g; K2HPO4, 1 g; KH2PO4, 0.5 g; MgSO4.7H2O, 1 g; yeast extract, pH 7.0; with shaking speed of 150 rpm for 4 days at 50 oC. The culture broth was obtained by filtration through filter paper (Whatman No. 1) and used for analyzed the PLA-degrading activity.