2.3. DNA extracted, PCR and sequencing
The total DNA extracted, PCR and sequencing were processed
according to Chen et al. (2012). Due to DNA extracted from 1 mg fish
tissue (or 0.2 ng/reaction) as limit of our PCR method. DNA was
extracted from a total of 20 mg of fresh fish, the commercial
products, and the handmade processed products using magnetic
beads (Chemagic DNA Tissue 10 Kit, Chemagen, Baesweiler, Germany)
and the Thermo Fisher Scientific KingFisher Magnetic Particle
Processor 5400000 Processor Kingfisher Mag Part