The blood samples were centrifuged

The blood samples were centrifuged and the plasma layer extracted
and frozen at 2808C until analysis. Free F2-isoprostanes in plasma were
quantified, after purification and derivatization, using GC/negative ion
chemical ionization-MS with [2
H4]8-iso-prostaglandin F2a as an internal
standard (20). Compounds were analyzed as trimethylsilyl ether
derivatives by monitoring mass-to-charge ratios of 569 and 573 for
endogenous F2-isoprostanes and the [2
H4]8-iso-prostaglandin F2a inter-
nal standard, respectively.

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The blood samples were centrifuged and the plasma layer extractedand frozen at 2808C until analysis. Free F2-isoprostanes in plasma werequantified, after purification and derivatization, using GC/negative ionchemical ionization-MS with [2H4]8-iso-prostaglandin F2a as an internalstandard (20). Compounds were analyzed as trimethylsilyl etherderivatives by monitoring mass-to-charge ratios of 569 and 573 forendogenous F2-isoprostanes and the [2H4]8-iso-prostaglandin F2a inter-nal standard, respectively.

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กลุ่มตัวอย่างเลือดปั่นและชั้นพลาสม่าที่สกัดและแช่แข็งที่ 2808C จนการวิเคราะห์
ฟรี F2-isoprostanes
ในพลาสมาถูกวัดหลังจากที่บริสุทธิ์และอนุพันธ์โดยใช้GC /
ไอออนลบสารเคมีไอออนไนซ์-MS ด้วย [2
H4] 8 ISO-prostaglandin F2A
เป็นภายในมาตรฐาน(20) สารประกอบที่ได้มาวิเคราะห์เป็นอีเทอร์ trimethylsilyl
สัญญาซื้อขายล่วงหน้าโดยการตรวจสอบอัตราส่วนมวลต่อการดูแลของ 569 และ 573
สำหรับภายนอกF2-isoprostanes และ [2
H4] 8 ISO-prostaglandin F2A
ระหว่างมาตรฐานNAL ตามลำดับ

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