Play VideoLimitationsSome organisms will not display sufficient growth in this medium to make an accurate determination, and additional follow-up testing is required.It is recommended that biochemical, immunological, molecular, or mass spectrometry testing be performed on colonies from pure culture for complete identification.False-negative reactions may occur if bacterial flagella are damaged due to heating, shaking, or other trauma. Such environmental shock will render the organism non-motile.Organisms which are weakly motile may result in false-negative reactions.When inoculating semi-solid media, it is important that the inoculating needle be removed along the exact same line used to inoculate the medium. A fanning motion may result in growth along the stab line that may result in false-positive interpretation.