deposited onadditionof MeOHwerefilt

deposited onadditionof MeOHwere
fi
ltered off,and the
fi
ltrate
was passed through a Sephadex LH-20 column (1.6×50 cm)
eluted with MeOH (200 ml; fraction of 10 ml) to afford twenty
fractions. The hydrolysates (fractions 10
–
13) were combined
on the basis of TLC behaviors, concentrated under vacuum and
the dried residue was chromatographed on silica gel column
(1.6×40 cm) with gradient CHCl
3
/MeOH (19:1 to 7:3; 1 l;
fraction of 10 ml) to afford the sapogenin
1a
(30 mg) and sugar
mixture (48 mg). The sapogenin
1a
(
Rf
=0.40) and the sugar
mixturewereobtainedfromthefractionsanalyzedbysilicagel-
TLC using the solvent systems (B) CHCl
3
/MeOH (95:5, v/v) and
(C)
n
-BuOH/Me
2
CO/H
2
O (4:5:1, v/v/v), respectively. A sample
of the sugar mixture (1 mg) was dissolved in pyridine (100
μ
l)
and analyzed by silica gel-TLC in the above described solvent
(C). After spraying with orcinol/H
2
SO
4
, glucose and galactose
gave blue spots at
Rf
values of 0.38 and 0.35, respectively.
(3
β
,5
α
,6
β
,25
R
)-Spirostan-3,6-diol (
1a
): Colorless needles
from benzene, mp 239
–
240 °C; [
α
]
D
25
–
72.5° (
c
0.5, CHCl
3
); IR
ν
max
(KBr): 3520, 3250 (OH), 2955, 2930, 2875, 2850 (CH),
1450, 1375, 1340, 1240, 1175, 1075, 1055, 1015, 1005, 980,
955, 915, 900, 860 cm
−
1
(intensity 915
b
900, 25
R
-spiroke-
tal); EIMS:
m
/
z
432 [M]
+
, 139 [C
9
H
15
O]
+
; The
1
H and
13
C
NMR (pyridine-
d
5
) data agreed well with those of literature
data

Results (Indonesian) 1:

deposit onadditionof MeOHwereFiltered, danFiltratedisahkan melalui kolom Sephadex LH-20 (1.6 × 50 cm)eluted dengan MeOH (200 ml; sebagian kecil dari 10 ml) mampu dua puluhpecahan. Hydrolysates (fraksi 10–13) digabungkanBerdasarkan perilaku TLC, terkonsentrasi di bawah vakum danresidu kering chromatographed pada kolom silika gel(1.6 × 40 cm) dengan gradien CHCl3/ MeOH (19:1 hingga 7:3; 1 l;sebagian kecil dari 10 ml) mampu sapogenin1a(30 mg) dan gulacampuran (48 mg). Sapogenin1a(RF= 0,40) dan gulamixturewereobtainedfromthefractionsanalyzedbysilicagel-TLC menggunakan sistem pelarut (B) CHCl3/ MeOH (95:5, v/v) dan(C)n-BuOH / saya2CO/H2O (4:5:1, v/v/v), masing-masing. Sampelgula campuran (1 mg) dibubarkan pada piridina (100Μl)dan dianalisis oleh silika gel-TLC dalam pelarut dijelaskan di atas(C). setelah penyemprotan dengan orcinol H2JADI4, glukosa dan galaktosamemberi bintik-bintik biru diRFnilai-nilai 0.38 dan 0,35, masing-masing.(3Β, 5Α, 6Β, 25R) - Spirostan - 3,6-diol ()1a): Berwarna jarumdari benzena, mp 239–240 ° C; [Α]D25–72.5°)c0,5, CHCl3); IRΝMax(KBr): 3520, 3250 (OH), 2955, 2930, 2875, 2850 (CH),1450, 1375, 1340, 1240, 1175, 1075, 1055, 1015, 1005, 980,955, 915, 900, 860 cm−1(intensitas 915b900, 25R-spiroke-Tal); EIMS:m/z432 [M]+, 139 [C9H15O]+; The1H dan13CNMR (piridina-d5) data setuju juga dengan orang-orang sastradata