Based on the reported therapeutic a

Based on the reported therapeutic activities of ursolic
acid (anticancer, anti-inflammatory, anti-oxidative and
hepatoprotective, etc.6), β-sitosterol (anticancer,
estrogenic, hypolipidemic, etc.7), lupeol (antiinflammatory,
anticancer, antimicrobial,
hepatoprotective, cadioprotective activity, etc.8) and
gallic acid (antioxidant, anti-allergic, anti-inflammatory,
anti-mutagenic, anti carcinogenic, apoptotic and wound
healing, etc.9); chromatographic characterization of C.
cainito leaves was carried out in terms of these
triterpenoids (ursolic acid, β-sitosterol and lupeol) and
phenolic compound (gallic acid) content. Further, in order
to evaluate safety of C. cainito leaves, acute oral toxicity
study (as per OECD guideline no. 420) was conducted in
Albino Wistar rats.
MATERIALS AND METHODS
Chemicals and reagents
Ursolic acid, β-sitosterol, lupeol and gallic acid (98 %
purity each) were procured from Sigma Aldrich Chemical
Company, (Steinheim, Germany). Chemicals of analytical
grade were purchased from Merck Specialties Private
Limited, Mumbai.

Results (Indonesian) 2:

Berdasarkan kegiatan terapi yang dilaporkan ursolic
acid (antikanker, anti-inflamasi, anti-oksidatif dan
hepatoprotektif, etc.6), β-sitosterol (antikanker,
estrogenik, hipolipidemik, etc.7), lupeol (antiinflamasi,
antikanker, antimikroba,
hepatoprotektif, aktivitas cadioprotective, etc.8) dan
asam galat (antioksidan, anti-alergi, anti-inflamasi,
anti-mutagenik, anti karsinogenik, apoptosis dan luka
penyembuhan, etc.9); karakterisasi kromatografi C.
cainito daun dilakukan dalam hal ini
triterpenoid (asam ursolic, β-sitosterol dan lupeol) dan
senyawa fenolik (asam galat) konten. Selanjutnya, dalam rangka
untuk mengevaluasi keselamatan C. cainito daun, toksisitas akut oral
studi (sesuai pedoman OECD tidak ada. 420) dilakukan pada
tikus Albino Wistar.
BAHAN DAN METODE
Kimia dan reagen
asam ursolat, β-sitosterol, lupeol dan asam gallic (98%
kemurnian masing-masing) yang diperoleh dari Sigma Aldrich Chemical
Company, (Steinheim, Jerman). Bahan kimia dari analisis
kelas yang dibeli dari Merck Specialties Swasta
Limited, Mumbai.