The maximum TC removal rate (50.74%) occurred in the 12th day after TC addition, then it decreased. The rate of this decrease slowed with time. In C2, the removal rate of TC ranged from 5.05–23.18% during operation. This rate also increased in the first few after TC exposure. The maximum TC removal rate (23.18%) occurred the 9th day after TC addition and then it slowly decreased and flattened out by the final 18 days of operation. In C3, the removal rate of TC ranged from 2.52–17.71%, It reached the maximum level (17.71%) on the 15th day, plateaued