Objective:
To determine the accuracy of the Accu-Check Advantage home glucose monitor, and related factors.
In this paper, a portable and sensitive DNA quantification method based on personal glucose meters and isothermal circular strand-displacement polymerization reaction was developed.
The capture DNA was fixed onto Streptavidin-MNBs. Then target DNA (in red color) hybridized with the capture DNA and opened their loop parts, enabling a primer with DNA–invertase conjugation to attach on the stem part, which initiated a polymerization which replaced the target with the aid of the DNA polymerase I.
The released target then found another capture DNA to trigger another polymerization cycle, which was repeated for many rounds, resulting in the multiplication of the DNA–invertase conjugation on the surface of Streptavidin-MNBs.
The bound DNA–invertase can be used to catalyze the hydrolysis of sucrose into glucose with millions of turnovers, which transformed the concentration of target DNA into the level of glucose for monitoring of PGM.