Manno-oligosaccharides (MOS) consist of a linear chain of mannose and have gainedgreat interest as a prebiotic. MOS can be derived from mannan-rich plants, such as coprameal, a by-product of coconut milk and coconut oil processing. In Thailand, 25 millionmetric tons of copra meal is produced annually [7]. By enzymatic hydrolysis using βmannanase, a copra meal hydrolysate (CMH) was obtained as a source for further MOSproduction. CMH is stable under human gastrointestinal tract conditions. CMH is relatively stable in the small intestine and is easily fermented by lactobacilli and bifidobacteria [8].Very recently, the impact of CMH on gastrointestinal symptoms and gut microbiome wasdemonstrated to have a positive relationship with health using 16S rRNA gene sequencing [9]. In the context of taxonomy and metabolic function-associated pathways, the humangut microbiome is largely unknown at the species level, as are the associated metabolicroutes involved in short-chain fatty acids (SCFAs).