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Biotechnology can complement conventional breeding and expedite the mango improvement programmes. Studies involving invitro culture and selection, micropropagation, embryo rescue, genetic transformation, marker-assisted characterization and DNAfingerprinting, etc. are underway at different centers worldwide. In vitro culture and somatic embryogenesis of several differentgenotypes have been achieved. The nucellus excised from immature fruitlets is the appropriate explant for induction of embryogeniccultures. High frequency somatic embryogenesis has been achieved in some genotypes; however, some abnormalitiescan occur during somatic embryo germination. Embryo rescue from young and dropped fruitlets can improve the hybridizationsuccess in a limited flowering season. Protocols for protoplast culture and regeneration have also been developed. In vitroselections for antibiotic tolerance and fungal toxin resistance have been very promising for germplasm screening. Genetictransformation using Agrobacterium tumefaciens has been reported. Genes that are involved with fruit ripening have been clonedand there have been attempts to deliver these genes into plants. DNA fingerprinting and studies on genetic diversity of mangocultivars and Mangifera species are also being conducted at several research stations. The purpose of this review is to focus uponcontemporary information on biotechnological advances made in mango. It also describes some ways of overcoming the problemsencountered during in vitro propagation of mang
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