For the flowers rust spot disease, harpin protein at both concentrations provided superior protection against the disease, with a statistically significant difference from B. subtilis AP-01 + T. harzianum AP-001, mancozeb 80% WP and the two control treatments (F = 40.8; df = 5,18; p < 0.05) (Figure 1a). However, the microbial control agents and the chemical fungicide still provided disease protection. Figure 2 illustrates the rust spot disease severity for each treatment after 28 days.