Th e relationship among antioxidant

Th e relationship among antioxidant enzyme activities, such as iron-superoxide dismutase (FeSOD) and
ascorbate-dependent peroxidase (AsA-POD), and phycocyanin, chlorophyll, total carotenoid, ascorbate contents, and
lipid peroxidation (LPO) levels were investigated in Spirulina maxima SAG 84.79 in nitrate limited (10 mM), control
(30 mM), and supplemented (50 mM) conditions. In the present study, extracellular nitrate and intracellular iron,
magnesium, and copper levels were also investigated. Th e highest FeSOD and AsA-POD activities and chlorophyll
content and the lowest LPO levels were found in control conditions on day 13: 21.9 ± 1.5, 5.7 ± 0.3 IU mg–1, 16.2 ±
0.5 mg g–1, and 0.322 ± 0.01 nmol malondialdehyde g–1 wet weight, respectively. Th e highest phycocyanin content was
determined in nitrate supplemented conditions on day 13, at 190.68 ± 9.3 mg g–1. Th e similar LPO levels observed
in supplemented (50 mM nitrate) and control (30 mM) conditions showed that phycocyanin demonstrated eff ective
protection of S. maxima membranes. Th e data revealed that its response against nitrate exposure was related to the
antioxidant enzymes and compounds in the antioxidant defence system of S. maxima.

Results (Indonesian) 2:

E hubungan Th antara aktivitas enzim antioksidan, seperti besi-superoxide dismutase (FeSOD) dan
askorbat tergantung peroksidase (ASA-POD), dan phycocyanin, klorofil, jumlah karotenoid, isi askorbat, dan
peroksidasi lipid (LPO) tingkat diselidiki di Spirulina maxima SAG 84.79 di nitrat terbatas (10 mM), kontrol
(30 mM), dan ditambah (50 mM) kondisi. Dalam penelitian ini, nitrat ekstraseluler dan besi intraseluler,
magnesium, dan tembaga tingkat juga diselidiki. Th e tertinggi FeSOD dan Asa-POD kegiatan dan klorofil
konten dan tingkat PUT terendah ditemukan dalam kondisi kontrol pada hari 13: 21,9 ± 1,5, 5,7 ± 0,3 IU mg-1, 16,2 ±
0,5 mg g-1, dan 0,322 ± 0,01 nmol malondialdehid g-1 berat basah, masing-masing. Th e konten phycocyanin tertinggi
ditentukan dalam kondisi dilengkapi nitrat pada hari 13, di 190,68 ± 9,3 mg g-1. Th e tingkat PUT serupa diamati
di ditambah (50 mM nitrat) dan kontrol (30 mM) kondisi menunjukkan bahwa phycocyanin menunjukkan efektif eff
perlindungan membran S. maxima. Data e th mengungkapkan bahwa respon terhadap paparan nitrat terkait dengan
enzim antioksidan dan senyawa dalam sistem pertahanan antioksidan S. maxima.