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Thai) 1:
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allyglycidyl ether (AGE), N,N-methylene-bis(acrylamide) (MBAA), and acrylamide (MAA), and used for isomerization of glucose to fructose. Effects of immobilization conditions and reaction conditions on theactivity ofimmobilized GI, the kinetic parameters, the operational stability, thermal stability and storagestability of immobilized GI were investigated. The optimum immobilization conditions were GI additionamount of 0.3 ml GI/g support, immobilization time of 24 h, and immobilization temperature of 25 ◦C.The optimum reaction conditions were pH value of reaction solution of 7.5 and reaction temperatureof 65 ◦C. The activity of immobilized GI was 450 U/g (wet). Km and Vmax values of immobilized GI were1.16 mol/L and 1.07 × 10−3 mol/L min, respectively. Immobilized GI onto GAMM support has better operational stability, thermal stability and storage stability, in which it retained 91% ofits initial activity afterrecycled for 18 times and retained 97% of its initial activity after stored at 4 ◦C for six weeks. Thereforeimmobilized GI onto GAMM support was an excellent catalyst for isomerization of glucose to fructose.
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