Abstract
A protocol for plantlet regeneration through
shoot formation was developed for the neotropical shrub
Brunfelsia calycina. This shrub is unique in its change in
flower color from dark purple to white. Explants from
young and mature leaves were incubated on MS medium
(pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations
of Indole-3-acetic acid (IAA) and 6-Benzyladenine
(BA) under a 16 h photoperiod at a constant temperature of
25C. Shoot emergence was best at 4.44 lM BA and
2.85 lM IAA for young leaf explants, and at 8.88 lM BA,
2.85 lM IAA for mature leaf explants. When shoots were
transferred to MS medium supplemented with 1.23–
2.46 lM indole butrytic acid (IBA), they developed roots.