oxidase enzymes play a vital role i

oxidase enzymes play a vital role in the operation of the electron transport system during aerobic respiration.Cytochrome oxidase catalyzez the oxidation of a reduced cytochrome by moleculer oxygen,resulting in the formation of h2o or h2o2.Aerobic bacteria,as well as some facultative anaerobes and microaerophiles,exhibit oxidase activity.The oxidase test aids in differentiation among members of the genera neisseria and pseudomonas,which are oxidase positive,and enterobacteriaceae,which ae oxidase negative.
The ability of bacteria to produce cytochome oxidase can be determined by the addition of the test reagent aminodimethylaniline oxalate to colonies growwn on a plate medium.This light pink reagent serves as an artificial substrate,donating electrons and thereby becoming oxidized to a blackish compound in the presence of the oxidase and free oxygen.Following the addition of the test reagent,the development of pink,then maroon,and finally dark purple coloration on the surface of the colonies is indicative of the absence of oxidase activity and is a negative test.

Results (Indonesian) 1:

enzim oksidase memainkan peran penting dalam pengoperasian sistem pengangkutan elektron selama aerobik respirasi. Catalyzez oksidase sitokrom oksidasi sitokrom dikurangi oleh oksigen moleculer, mengakibatkan pembentukan h2o atau h2o2. Bakteri aerobik, serta beberapa fakultatif anaerobes dan microaerophiles, memamerkan aktivitas oksidase. Tes oksidase aids di diferensiasi antara anggota genus neisseria dan pseudomonas, yang oksidase positif, dan enterobacteriaceae, oksidase ae yang negatif.Kemampuan bakteri untuk memproduksi cytochome oksidase dapat ditentukan dengan penambahan tes reagen aminodimethylaniline oksalat ke koloni growwn pada medium piring. Reagen merah muda cahaya ini berfungsi sebagai substrat buatan, menyumbangkan elektron dan dengan demikian menjadi teroksidasi untuk senyawa kehitaman hadapan oksidase dan oksigen bebas. Setelah penambahan test reagent, pengembangan merah muda, kemudian merah marun, dan akhirnya gelap ungu warna pada permukaan koloni-koloni indikasi adanya oksidase aktivitas dan tes yang negatif.