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Dyed substrates are particularlyuseful in developing zymograms from electrophoresis gels (Biely et al. 1985a,1988). As with all unnatural or substituted substrates, care must be taken toassure that the assay reflects the activity sought. Methods used to assayxylanase activities in various laboratories around the world were summarizedby Ghose and Bisaria (1987).
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