This study was conducted to evaluate the potential of betaine, taurine, inosine (INO), inosine 50monophosphate disodium (IMP Na2), and guanosine 50-monophosphate disodium (GMP Na2) as a feeding stimulant for juvenile marble goby (Oxyeleotris marmoratus) (total length 6.6–8.5 cm) through behavioural assays using agar gel pellets. All fish were conditioned to accept agar gel pellet before the behavioural assays started. Each chemical substance was tested on 50 replicates of individual fish once, and the overall ingestionrate was calculated as the representative data. The pure agar gel pellet was totally rejected by the fish (0 % ingestion rate).