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The toxic action of OPs by inhibition of catalytic activity ofacetylcholinesterase (AChE) prevents nerve transmission by blockingbreakdown of the transmitter choline. Biosensors based on theinhibition to AChE have been widely used for the detection of OPand carbamate pesticides. Du et al. (2007) reported an AChEbiosensor for rapid determination of triazophos, an organophophorusinsecticide. A multiwalled carbon nanotube (MWNT)–chitosancomposite was used as the working electrode and AChE was noncovalentlyimmobilized on the electrode surface using glutaraldehydeas the cross-linker. The interaction of AChE with
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