Purslane extract did not affect coliform and Escherichia coli populations but increased Lactobacillus population of cecal content significantly (p ≤ 0.05).
There were no significant differences in primary and secondary antibody titer against SRBC and no differences among the treatments for relative weight of thymus and spleen (p > 0.05).
Relative weight of bursa was affected with inclusion of purslane extract in the diet.
Therefore, it was concluded that purslane inclusion had a positive significant effect on cecal microflora composition, but had no effect on immune response of broiler chickens.