Cloning and ExpressionBy database s

Cloning and Expression
By database searching for GPCR-like sequences, followed by RACE, Oda et al. (2000) obtained a cDNA encoding HRH4, which they termed GPRV53. The deduced 390-amino acid protein is almost 40% identical to HRH3 (604525). It contains an asp residue in transmembrane domain-1 (TM1), a DRY motif in TM2, a disulfide bond between the first and third extracellular loops, a trp in TM4 and TM6, a pro in TM5 and TM6, an NPxxY motif in TM7, and a potential palmitoylation site in the C-terminal tail. Northern blot analysis revealed expression of a 4.5-kb transcript restricted to peripheral blood leukocytes. RT-PCR analysis detected expression in thymus, small intestine, spleen, and colon. No expression was detected in brain, where HRH3 expression is restricted. Within blood, expression was most abundant in eosinophils.

Results (Indonesian) 2:

Kloning dan Ekspresi
Dengan database yang mencari urutan GPCR-seperti, diikuti oleh RACE, Oda et al. (2000) memperoleh cDNA encoding HRH4, yang mereka sebut GPRV53. Protein asam 390-amino yang disimpulkan hampir 40% identik dengan HRH3 (604.525). Ini berisi residu asp di transmembran domain-1 (TM1), motif KERING di TM2, ikatan disulfida antara loop ekstraseluler pertama dan ketiga, trp di TM4 dan TM6, pro dalam TM5 dan TM6, motif NPxxY di TM7 , dan situs palmitoylation potensial di ekor C-terminal. Analisis Northern blot mengungkapkan ekspresi transkrip 4.5-kb terbatas leukosit darah perifer. RT-PCR analisis terdeteksi ekspresi dalam timus, usus kecil, limpa, dan usus besar. Tidak ada ekspresi terdeteksi di otak, di mana ekspresi HRH3 dibatasi. Dalam darah, ekspresi paling melimpah di eosinofil.