1.3 mM 2-mercaptoetanol and 3.2 mM l-cysteine hydrochloride were the reducing agents that generated higher Eh reductions and “Eh buffer effects” in 100 mM YNB-glucose broth; (b) 2-mercaptoetanol, when in anoxic atmosphere, reduces significantly the growth rate; (c) addition of oxidizing agents does not stimulate candidal growth rate; (d) addition of reducing or oxidizing agents and posterior incubation in normoxic or anoxic atmospheres do not cause alterations in electrophoretic profiles of whole-cell proteins; and (e) CSH is extensively altered when C. albicans is submitted to above mentioned treatments.