oxidase enzymes play a vital role i

oxidase enzymes play a vital role in the operation of the electron transport system during aerobic respiration.Cytochrome oxidase catalyzez the oxidation of a reduced cytochrome by moleculer oxygen,resulting in the formation of h2o or h2o2.Aerobic bacteria,as well as some facultative anaerobes and microaerophiles,exhibit oxidase activity.The oxidase test aids in differentiation among members of the genera neisseria and pseudomonas,which are oxidase positive,and enterobacteriaceae,which ae oxidase negative.
The ability of bacteria to produce cytochome oxidase can be determined by the sddition of the test reagent aminodimethylaniline oxalate to colonies growwn on a plate medium.This light pink reagent serves as an artificial substrate,donating electrons and thereby becoming oxidized to a blackish compound in the presence of the oxidase and free oxygen.Following the addition of the test reagent,the development of pink,then maroon,and finally dark purple coloration on the surface of the colonies is indicative of the absence of oxidase activity and is a negative test.

Results (Indonesian) 2:

enzim oksidase memainkan peran penting dalam pengoperasian sistem transpor elektron selama aerobik respiration.Cytochrome oksidase catalyzez oksidasi sitokrom dikurangi dengan oksigen molekuler, menghasilkan pembentukan H2O atau bakteri h2o2.Aerobic, serta beberapa anaerob fakultatif dan mikroaerofil, bantu tes pameran oksidase activity.The oksidase dalam diferensiasi antara anggota neisseria genera dan pseudomonas, yang oksidase positif, dan enterobacteriaceae, yang oksidase negatif ae.
kemampuan bakteri untuk menghasilkan cytochome oksidase dapat ditentukan oleh sddition dari uji reagen aminodimethylaniline oksalat ke koloni growwn pada reagen merah muda piring medium.This cahaya berfungsi sebagai substrat buatan, menyumbangkan elektron dan dengan demikian menjadi teroksidasi menjadi senyawa kehitaman di hadapan oksidase dan oxygen.Following gratis penambahan reagen tes, pengembangan pink, kemudian merah marun, dan warna ungu akhirnya gelap pada permukaan koloni merupakan indikasi tidak adanya aktivitas oksidase dan merupakan tes negatif.