As a whole, CS and 3HAD activities of lion and caracal muscles were approximately 3.5× and 2.5× lower, respectively, than activities measured in human athletes (Table 1). Surprisingly, PFK activities of both felid species were lower than that of human muscle, and different from each other. On the other hand, LDH activity of lion and caracal was 4× and 6× higher, respectively, than activities measured in human athletes. The pathways representing high-energy phosphates (CK) and glycogen breakdown (PHOS) were approximately 2× higher in the animals compared to humans