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Tinjauan ini, wefocusedontheuseofantimicrobialtestingmetode untuk investigationofextractsandpuredrugsas secara in vitropotentialantimicrobialagents.Setelah therevolutioninthe "zaman keemasan", whenalmostallgroupsdari importantantibiotics (tetracyclines, sefalosporin, aminogly-werediscoveredandthemainproblems cosides andmacrolides)dari chemotherapyweresolvedinthe1960s, thehistoryrepeatsitu sendiri nowadaysandtheseexcitingcompoundsareindangerofkehilangan theirefficacy becauseoftheincreaseinmicrobialresistance[1]. saat ini, itsimpactisconsiderablewithtreatmentfailuresassociatedwithmultidrug-resistantbacteriaandithasbecomeaGlobal concerntopublichealth [2,3]
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