Recovery studiesTo study the accura

Recovery studies
To study the accuracy and precision of the method, recovery
studies were performed by the method of standard addition.
The recovery of added standard was studied at three different levels,
each being analyzed in a manner similar to that described for
the assay (40–42). The methanol extract of CP, EA, CT and CD
were used for recovery studies. These were preanalyzed by the
developed method as mentioned in the experimental sections
and found to contain 134.2 and 146.1 mg of ursolic acid per
gram of CT and CD; 110.6 mg of betulinic acid per gram of EA;
92.75, 154.95, 31.947 and 39.21 mg of stigmasterol per gram of
EA, CP, CT and CD and 30.12 mg of lupeol per gram of CT, respectively.
Thus, 20 mg of the same preanalyzed extracts contained
2684 and 2922 ng/spot of ursolic acid in CT and CD;
2212 ng/spot of betulinic acid in EA; 1855, 3099, 638.94 and
784.2 ng/spot of stigmasterol in EA, CP, CT and CD and
602.4 ng/spot of lupeol in CT, respectively, which were used
for the recovery studies. The preanalyzed extract samples of all
were spiked with an extra 1:2 ratio, i.e., 100 and 200 ng/spot
of the respective standard of the ursolic acid, betulinic acid, stigmasterol
and lupeol content of the respective sample, which
were reanalyzed by the proposed method.

Results (Indonesian) 2:

Studi pemulihan
Untuk mempelajari akurasi dan presisi metode, pemulihan
penelitian dilakukan dengan metode penambahan standar.
Pemulihan standar menambahkan dipelajari pada tiga tingkat yang berbeda,
masing-masing yang dianalisis dengan cara yang sama dengan yang dijelaskan untuk
pengujian tersebut (40 -42). Ekstrak metanol CP, EA, CT dan CD
yang digunakan untuk studi pemulihan. Tersebut preanalyzed dengan
metode yang dikembangkan seperti yang disebutkan di bagian eksperimental
dan ditemukan mengandung 134,2 dan 146,1 mg asam ursolic per
gram CT dan CD; 110,6 mg asam betulinic per gram dari EA;
92,75, 154,95, 31,947 dan 39,21 mg stigmasterol per gram
. EA, CP, CT dan CD dan 30,12 mg lupeol per gram dari CT, masing-masing
demikian, 20 mg sama preanalyzed ekstrak yang terkandung
2684 dan 2922 ng / tempat asam ursolic di CT dan CD;
2212 ng / tempat asam betulinic di EA; 1855, 3099, 638,94 dan
784,2 ng / spot stigmasterol di EA, CP, CT dan CD dan
602,4 ng / spot lupeol di CT, masing-masing, yang digunakan
untuk studi pemulihan. Sampel ekstrak preanalyzed dari semua
dibubuhi dengan tambahan 1: 2 rasio, yaitu, 100 dan 200 ng / tempat
dari standar masing-masing asam ursolic, asam betulinic, stigmasterol
dan konten lupeol dari masing-masing sampel, yang
dianalisis kembali oleh metode yang diusulkan.