HPTLC studies were carried out foll

HPTLC studies were carried out following the method
of Harborne[12] and Wagner et al[13]. The protocol for
preparing sample solutions was optimized for high quality
fingerprinting. The fingerprinting of active chloroform
fractions F1, F2, F4, F5, F7, F9, F11 and F14 were executed by
spotting 10 μL of suitably diluted sample solution on a HPTLC
plate. The plates were developed and scanned. The peak
areas were recorded. Chromatography was performed on 20
cm伊10 cm glass HPTLC plates precoated with 200 μm layers
of silica gel 60F254 (E. Merck, Darmstadt, Germany). Sample
of each fraction was applied as bands 6 mm wide and 8 mm
apart by means of CAMAG (Muttenz, Switzerland) Linomat IV
sample applicator equipped with a 25 μL syringe which was
programmed through WIN CATS software

Results (Indonesian) 1:

HPTLC studi dilakukan mengikuti metodeHarborne [12] dan Wagner et al [13]. Protokol untukmempersiapkan solusi sampel telah dioptimalkan untuk kualitas tinggisidik jari. Sidik jari kloroform aktifpecahan F1, F2, F4, F5, F7, F9, F11 dan F14 dieksekusi olehSimak 10 μL larutan diencerkan sesuai sampel HPTLCpiring. Pelat dikembangkan dan scan. Puncakdaerah tercatat. Kromatografi dilakukan pada 20cm伊10 cm kaca HPTLC piring precoated dengan 200 μm lapisandari silika gel 60F254 (E. Merck, Darmstadt, Jerman). Sampelsetiap fraksi diaplikasikan sebagai band lebar 6 mm dan 8 mmterpisah oleh berarti IV Linomat CAMAG (Muttenz, Swiss)sampel aplikator dilengkapi dengan 25 μL jarum suntik yangdiprogram melalui perangkat lunak MEMENANGKAN KUCING