The use of natural ingredients such as herbs had a tremendous growth in food preservation. These compounds, in
addition to antimicrobial properties, have flavoring and antioxidant properties as well. Recent research suggests that
certain live microorganisms may play a role in regulating the immune system and cancer prevention that of among
them to the probiotic bacteria can be noted. On the other hand, probiotic products by improving the intestinal
microbial flora and digestive tract, and boost the immune system have significant impact on consumers' health. This
study was conducted to evaluate the effect of aqueous and ethanolic extracts of Purslane on activities of probiotic
bacteria Lactobacillus casei strain T4 and Lactobacillus acidophilus strain LA-5. The purpose of this study was to
evaluate the effect of Purslane on the growth of Lactobacillus bacteria hence was prepared the aqueous and
ethanolic extracts of Purslane and probiotic bacteria were exposed to various concentrations (2.5, 5 and 7%)of
aqueous and ethanolic extracts. The results were evaluated after 24 hours by spectrophotometry and optical density
(OD) methods. The results showed that some concentrations (2.5 and 5 %) of ethanol extract of Purslane and only
the 7% concentration of aqueous extracts of purslaneincrease the growth of LA-5, whereas, the aqueous extract and
also ethanolic extract of purslane at certain concentrations, can reduce the growth of T4