Hence, this work was undertaken to evaluation of oreganoessential oil effect, in different proportions, on the properties of biocomposite films. The characterizations included water barrierability, mechanical behavior, optical properties, microstructural,and surface morphology. The antimicrobial properties of the filmsagainst gram-positive bacteria (Staphylococcus aureus and Listeriamonocytogenes) and gram-negative bacteria (Salmonella enteritidisand Escherichia coli) were also assessed.