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3.9 การคุณสมบัติต้านจุลชีพThe antimicrobial activity of biocomposite films incorporated with oregano oil at various contents against the selected microorganisms is presented in Table 3. The control film (without OEO)showed any antimicrobial effect against all 4 studied microorganisms, resulting in no inhibition zones (Table 3). The results are in concordance with Gómez-Estaca et al. (2010) who reported that gelatin–chitosan edible films showed no antibacterial activity against E. coli, Pseudomonas fluorescens, Lactobacillus acidophilusand Listeria innocua. According to Coma et al. (2002) chitosan is unable to diffuse through the adjacent agar media; as only organisms in direct contact with the active sites of chitosan are prevented.It was also suggested that chitosan only in a gelled or viscous acid solution forms exhibit antimicrobial properties (Fernandez-Saiz et al., 2009). Under these conditions, the biopolymer amine groups are protonated or “activated”.Biocomposite films incorporated with 0.8% (w/v) OEO showed the certain inhibitory effect against S. aureus, L. monocytogenes, andS. enteritidis, while the slight inhibition was found for the E. coli.The minimum concentration of OEO at which the films begin toexhibit antibacterial activity agree with that found by Benavideset al. (2012), who have reported that the incorporation of 1% (w/v)oregano essential oil into alginate films shows antibacterial effect against E. coli, S. enteritidis, S. aureus, and L. monocytogenes.The investigation presented here demonstrated that the high estessential oil concentration (i.e. 1.2% w/v), was effectively inhibited the growth of tested microorganisms, producing halos between
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