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particle doesn't affect the current between electrodes but causes a voltage pulse that is proporsional to the resistance change and the particle volume. The voltage pulses are amplified and passed through a pulse height analyser with variable threshold control which is calibrated in terms of particle size. the treshold setting is such that only resistances over a certain value are counted. Tge treshold setting is then changed and corresponding reading for larger particles noted. In this fashion an oversize curve is generated. It should be noted that the distribution one gets by the Coulter counter is a number distributer that is, it counts each particle, not its size, as long as it is over the treshold value. The total number of particles is estimated such as the distribution will be logarithmacally linear on the probability curve.
The sample of suspension used in the Coulter counter should be sufficiently dilute so that only one particle at a time passes through the aperture.
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