women with a negative or indeterminate history of varicella have been exposed significantly to VZV by household contact, face-to-face contact for at least 5 minutes or indoors contact for more than 15 minutes, virus-specific IgG class antibodies should be measured immediately. If the woman is seronegative or there is an indeterminate or unknown status of immunity, she should be regarded as susceptible. Antibodies detected within 7-10 days of contact must have been acquired before exposure. For pregnant women, who were adequately vaccinated with 2 vaccine doses, routine serologic test-ing cannot be recommended since 99% of persons be-come seropositive after the second dose of varicella vac-cination [44]. Furthermore, most currently used enzyme immunoassays are too insensitive to detect vaccine-induced VZV-specific IgG class antibodies [45] and sensitive procedures such as the fluorescent antibody to mem-brane antigen assay or tests for the determination of the cell-mediated immune response are too laborious and/or time consuming for daily routine. Recently, a new gen-eration of VZV glycoprotein-based enzyme immunoas-says has been shown to have higher sensitivity for the determination of VZV IgG [46]