2.5. Extracts preparation
After baking, bread samples were allowed to cool down to room temperature for 24 h. Subsequently, the samples were sliced (slices about 1.5 cm thick). The crust was removed aseptically and kept frozen (at 20 C) until analysis. After thawing, the slices were dried and then manually crumbed, ground in a mill and screened through a 0.5 mm sieve to obtain bread powder.