Results (
Thai) 1:
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Fungi metabolise organic matter in situ and so alter both the bio-/physico-chemical properties andmicrobial community structure of the ecosystem. In particular, they are responsible reportedly forspecific stages of decomposition. Therefore, this study aimed to extend previous bacteria-based forensicecogenomics research by investigating soil fungal community and cadaver decomposition interactionsin microcosms with garden soil (20 kg, fresh weight) and domestic pig (Sus scrofa domesticus) carcass(5 kg, leg). Soil samples were collected at depths of 0–10 cm, 10–20 cm and 20–30 cm on days 3, 28 and77 in the absence (control Pg) and presence (experimental +Pg) of Sus scrofa domesticus and used fortotal DNA extraction and nested polymerase chain reaction and denaturing gradient gel electrophoresis(PCR–DGGE) profiling of the 18S rRNA gene. The Shannon–Wiener (H0) community diversity indiceswere 1.25 0.21 and 1.49 0.30 for the control and experimental microcosms, respectively, whilecomparable Simpson species dominance (S) values were 0.65 0.109 and 0.75 0.015. Generally, and incontrast to parallel studies of the bacterial 16S rRNA and 16S rDNA profiles, statistical analysis (t-test) of the18S dynamics showed no mathematically significant shifts in fungal community diversity (H0; p = 0.142) anddominance (S; p = 0.392) during carcass decomposition, necessitating further investigations
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