Results (
Thai) 2:
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A one-step immunochromatographic strip method for on-site
detection of AFB1 in food and feed samples was constructed
using antibodies specific to AFB1. This method assumes that
aflatoxins in the sample compete with AFB1eOVA conjugate for
binding with the colored antibodyegold nanoparticles. A situation
in which the aflatoxins concentration in the sample exceeds
a specified amount implies that the toxin occupies all binding
sites of antibodyegold conjugates in the “conjugated pad” area.
Consequently, in terms of the test zone, no free antibodyegold
conjugate is available for binding with AFB1eOVA. Therefore,
the red line on the test zone is absent, indicating a positive result.
Next, in this study whether the assay performed properly is
verified by constructing a control zone with a goat-anti-rabbit
secondary antibody. Under accurate operations, the control
zone should always display a red color line, regardless of the
presence or absence of aflatoxins. Therefore, an aflatoxin-free
sample should display two red lines on the membrane,
whereas a toxin positive sample should have only one red line
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