The remarkable spatial change of bacterial community structure have been found in waters and sediments of full-scale HSF-CW systems treating river water. also reported the remarkable depth-related change of bacterial community structure in pilot-scale surface water ceramic VF-CW. Moreover, found that bacterial community structure showed a considerable depth-related variation in both zeolite and sand VF-CW systems, but experienced a slight change in gravel VF-CW system. In the present study, the result of UPGMA clustering showed a considerable depth-related change of bacterial community structure in each VF-CW system. To date, the influential factors regulating bacterial community structure in surface water CW system remain unclear. suggested that VF-CW bacterial community structure could be significantly influenced by both substrate type and wetland depth. This was further sustained by the result obtained in the current study.