A total of 18 crossbred (Duroc Landrace Yorkshire) weaning
piglets (weaned on d 23, initial body weight 7.42 ± 0.19 kg) were
randomly assigned to 3 experimental groups (6 pigs per treatment,
half males and half females): control group, K88 challenged group
and CGMP treated group. Control group and K88 challenged group
were fed a cornesoybean basal diet, and CGMP treated group was
fed the basal diets supplemented with 1% CGMP. All piglets had ad
libitum access to feed and water throughout the 12-day trial. All
weaning pigs were fed the basal diet for 3 days as pretreatment. In
days 8e10, 30 mL of 10% (w/v) NaCHO3 and 20% (w/v) sucrose
solution was orally administered to all of the piglets, and 30 min
later, 30 mL of LB broth containing 109 CFU/mL of E. coli K88 was
inoculated to the K88 challenged group and CGMP treated group,
while 30 mL of sterile LB broth was inoculated to the control group.
The average daily gain (ADG), average daily feed intake (ADFI), and
feed/gain (F/G) of each pig were monitored throughout the experimental
period. The fecal scores were graded by Castillo et al.
(2008) (0 ¼ normally shaped feces, 1 ¼ shapeless feces, 2 ¼ soft
feces, and 3 ¼ thin, liquid feces).
The cornesoybean meal basal diet was formulated to meet the
nutrient requirements according to NRC (2012). (Table 1).