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Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum (RS) is one of the most serious tobaccodiseases worldwide and no effective control measures are available to date. This study investigated thepotential of Trichoderma harzianum SQR-T037 amended bioorganic fertilizer (BOF) and the arbuscularmycorrhizal fungi (AMF) Glomus mosseae 171 (Gm) on the control of TBW and promotion of plant growthin pot experiments. The results showed that the disease incidence in plants treated with integrated applicationof G. mosseae 171 and T. harzianum SQR-T037 amended bioorganic fertilizer (Gm + BOF) was thelowest, with a control efficacy of 68.2%, which is greater than that of BOF or Gm alone (26.8% and14.7%, respectively). The application of BOF or Gm alone significantly reduced the abundance of RS in rhizospheresoil, but the integrated treatment (Gm + BOF) showed the strongest inhibitory effect (with a21.3% increase in inhibition). The root colonization of G. mosseae 171 in samples treated with Gm+ BOF was higher than that in samples with solely Gm treatment, indicating that the BOF significantlypromoted G. mosseae mycorrhizal colonization. The results showed that the G. mosseae also had a positiveeffect on SQR-T037 rhizospheric colonization. Denaturing gradient gel electrophoresis (DGGE) resultsshowed that application of BOF and Gm alone or in combination changed the diversity of the rhizosphericmicrobial community. The integrated application of Gm + BOF to tobacco plants significantly increasesthe activity of polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), and peroxidase (POD),enzymes associated to systemic resistance. Additionally, the integrated application of Gm with BOFincreased the tobacco plant height, shoot dry weight, and root dry weight. In conclusion, a synergisticbiological approach integrating application of Gm and BOF for TBW protection seems promising.
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