We separately assessed whether nest building deficits appeared during two time periods ofacute ethanol withdrawal: 1) during the initial day of withdrawal when signs of acute ethanolwithdrawal have been previously observed (10 and 24 h after injection of ethanol), and 2)during the second day of withdrawal when these signs typically subside (28, 32 and 48 hafter injection). The details of this time course are described in the Introduction. Whensignificant interactions were found, lower order ANOVAs were conducted and significantinteractions pursued to the level of individual comparisons conducted with Bonferronicorrections. Distributions of the horizontal and vertical activity data during the posttreatment period from Experiment 4 were non-normal, with positive skews. These data werenormalized using a square-root transformation prior to statistical analysis, and data arepresented graphically as transformed. All significant results (p < 0.05) are reported.