We have been reported a strain of Streptomyces lucidus, an environmental isolate that produced some antibacterial agents and inhibit the growth of a number of pathogenic bacteria. Bioactive crude from. Streptomyces lucidus could show anti-methicillin resistant Staphylococcus aureus (MRSA) activity and gave more potent than oxacillin [34]. In drug discovery, antimicrobial agents arewere the first identification which determine whether they have clinically relevant antimicrobial activity, then tested for toxicity, and finally tested for their ability to be delivered to the site of infection. Therefore, the objective of this paper was aimed to (i) characterize the corresponding anti-MRSA agent