Moisture content of 70% was made up using the Mandel weber medium. Initially, pH of the medium was adjusted to 4.5. Subsequently, flasks were inoculated with 10 % of spore suspension containing 10'spores/ml and incubated at 45°C for 120 h. After cultivation of fungal isolate in the fermentation medium, 50 ml sodium citrate buffer (pH 4.8) was added and the cultures were centrifuged at 5000 rpm for 8 min at 4 °C