Microdilution method described by N

Microdilution method described by National Committee of Clinical
Laboratory Standards (NCCLS, 1993) was used for the standardization of
each plant part extract. Six clean test tubes were arranged in a rack and
labelled serially. To the first test tube, 4ml of dilute Dimethylsulphuoxide
(DMSO) (20 % DMSO in 80% distilled water) was added. Then from the
second test tube to the six, 2 ml of the dilute DMSO was added into each.
DMSO was chosen because of its high solvent power and selectivity. To
test tube one, 1 g of the already prepared plant part extract (paste) was put
and made to dissolve and mix well in it and 2 ml was pipetted from it to
test tube two already containing 2 ml of dilute DMSO. Again, 2 ml was
taken from test tube two and put into test tube three also containing 4 ml
of dilute DMSO. In this manner, the serial dilutions were done to test
tube six and 2 ml was discarded from the sixth test tube. The serial
dilution was in two-fold, i.e. 1/2 or 2-1, 1/4 or 4-1, 1/16 or 16-1, etc. Tube 2
to 6 contains 2 ml of diluent each. The purpose of the serial dilution of
the plant parts extract (paste) was to get different concentrations of it. The
concentrations obtained from the above serial dilution were in the order
test tube 1 to 6, i.e. 1 g/4ml or 250 mg/ml, 0.5 g/2 ml or 125 mg/ml, 0.25
g/2 ml or 62.5 mg/ml etc.

Results (Indonesian) 2:

Metode mikrodilusi dijelaskan oleh Komite Nasional Clinical
Laboratory Standards (NCCLS, 1993) digunakan untuk standarisasi
setiap bagian tanaman ekstrak. Enam tabung reaksi bersih yang disusun di rak dan
diberi label serial. Untuk tabung tes pertama, 4ml encer Dimethylsulphuoxide
(DMSO) (20% DMSO di 80% air suling) ditambahkan. Kemudian dari
tabung tes kedua dengan enam, 2 ml DMSO encer ditambahkan ke masing-masing.
DMSO dipilih karena daya pelarut tinggi dan selektivitas. Untuk
menguji tabung satu, 1 g bagian tanaman ekstrak sudah disiapkan (paste) dimasukkan
dan dibuat untuk melarutkan dan aduk di dalamnya dan 2 ml dipipet dari itu untuk
menguji tabung dua sudah mengandung 2 ml encer DMSO. Sekali lagi, 2 ml
diambil dari uji tabung dua dan dimasukkan ke dalam tabung reaksi tiga juga mengandung 4 ml
encer DMSO. Dengan cara ini, pengenceran serial dilakukan untuk menguji
tabung enam dan 2 ml dibuang dari tabung uji keenam. Serial
pengenceran berada di dua kali lipat, yaitu 1/2 atau 1/2, 1/4 atau 1/4, 1/16 atau 16-1, dll Tabung 2
ke 6 berisi 2 ml pengencer setiap. Tujuan dari pengenceran serial
bagian tanaman ekstrak (paste) adalah untuk mendapatkan konsentrasi yang berbeda itu. The
konsentrasi yang diperoleh dari seri pengenceran di atas berada di urutan
tabung 1 sampai 6, yaitu 1 g / 4ml atau 250 mg / ml, 0,5 g / 2 ml atau 125 mg / ml, 0,25
g / 2 ml atau 62,5 mg / ml dan lain-lain