Test MethodTest and control wound d

Test Method
Test and control wound dressings (n=16, 25 x 25 mm) were evenly distributed on to
the surface of 10mm thick nutrient agar made from tryptone (1.0% w/v), yeast extract
(0.5% w/v) (TYE) and pure agar (Oxoid Ltd, Basingstoke, UK) at a concentration of
1.0% (w/v). Media was sterilised, poured and set within large square assay plates
(235mm x 235mm). For some experiments the nutrient components were replaced by
PBS in the same strength agar. Test hydrogel wound dressing samples comprised the
iodide + glucose wound contact layer and the enzyme surface layer placed on top,
whereas the control hydrogel wound dressing samples (control 1) comprised only the
iodide + glucose wound contact layer without the surface enzyme layer (thus
preventing generation of hydrogen peroxide and iodine). Sample positions were
determined by reference to a grid matrix (intersection of rows and columns) and the
sequence of samples determined according to latin square principles. The test plates
loaded with dressing samples were pre-incubated for two hours to allow for initial gel
swelling of about 20 % (w/w) and the establishment of reaction-diffusion
equilibration of ROS flux prior to the introduction of the test disks containing
equivalent numbers of target species. These microbe-laden test disks were placed
between the nutrient agar layer and topical wound dressings of both test and control,
the components being manipulated with sterile forceps. Uncovered disks (n=16) were
also placed onto the test plates containing equivalent numbers of target species as an
additional uncovered control (control 2). Small metal weights (12 g) on nylon mesh
pads were then applied onto the top surface of each hydrogel square in order that
contact between gel, cellulose disk and agar surface was not disturbed with further gel
swelling (of approximately 20% over 12 h), following the initial equilibrium stage.
The large plate assay with disks and gels was incubated at 25°C for the duration of the
experiments.

Results (Indonesian) 1:

Test MethodTest and control wound dressings (n=16, 25 x 25 mm) were evenly distributed on tothe surface of 10mm thick nutrient agar made from tryptone (1.0% w/v), yeast extract(0.5% w/v) (TYE) and pure agar (Oxoid Ltd, Basingstoke, UK) at a concentration of1.0% (w/v). Media was sterilised, poured and set within large square assay plates(235mm x 235mm). For some experiments the nutrient components were replaced byPBS in the same strength agar. Test hydrogel wound dressing samples comprised theiodide + glucose wound contact layer and the enzyme surface layer placed on top,whereas the control hydrogel wound dressing samples (control 1) comprised only theiodide + glucose wound contact layer without the surface enzyme layer (thuspreventing generation of hydrogen peroxide and iodine). Sample positions weredetermined by reference to a grid matrix (intersection of rows and columns) and thesequence of samples determined according to latin square principles. The test platesloaded with dressing samples were pre-incubated for two hours to allow for initial gelswelling of about 20 % (w/w) and the establishment of reaction-diffusionequilibration of ROS flux prior to the introduction of the test disks containingequivalent numbers of target species. These microbe-laden test disks were placedbetween the nutrient agar layer and topical wound dressings of both test and control,the components being manipulated with sterile forceps. Uncovered disks (n=16) werejuga ditempatkan ke piring tes yang mengandung setara jumlah spesies target sebagaitambahan terungkap control (kontrol 2). Kecil logam berat (12 g) pada nylon meshbantalan yang kemudian diterapkan ke atas permukaan setiap persegi hydrogel agarkontak antara gel, selulosa permukaan disk dan agar tidak terganggu dengan gel lebih lanjutpembengkakan (kira-kira 20% lebih dari 12 h), setelah tahap awal keseimbangan.Piring besar assay dengan disk dan gel diinkubasi pada 25° C selamapercobaan.

Results (Indonesian) 2:

Metode tes
uji dan kontrol luka dressing (n = 16, 25 x 25 mm) yang merata pada
permukaan 10mm agar nutrien tebal yang terbuat dari tryptone (1,0% w / v), ekstrak ragi
(0,5% b / v) ( TYE) dan murni agar (Oxoid Ltd, Basingstoke, UK) pada konsentrasi
1,0% (w / v). Media itu disterilkan, dituangkan dan ditetapkan dalam persegi piring assay besar
(235mm x 235mm). Untuk beberapa eksperimen komponen nutrisi digantikan oleh
PBS di agar kekuatan yang sama. Uji hidrogel luka sampel saus terdiri atas
lapisan iodida + glukosa kontak luka dan lapisan permukaan enzim ditempatkan di atas,
sedangkan kontrol hidrogel luka sampel saus (kontrol 1) terdiri hanya
iodida + luka glukosa lapisan kontak tanpa lapisan enzim permukaan (dengan demikian
mencegah generasi hidrogen peroksida dan yodium). Posisi sampel yang
ditentukan dengan mengacu pada matriks grid (persimpangan baris dan kolom) dan
urutan sampel ditentukan sesuai dengan prinsip-prinsip persegi latin. Piring uji
sarat dengan sampel saus pra-diinkubasi selama dua jam untuk memungkinkan gel awal
pembengkakan sekitar 20% (b / b) dan pembentukan reaksi-difusi
equilibrium ROS fluks sebelum pengenalan disk uji yang mengandung
setara jumlah spesies sasaran. Disk uji mikroba-sarat ini ditempatkan
antara lapisan agar nutrien dan dressing luka topikal dari kedua uji dan kontrol,
komponen yang dimanipulasi dengan forsep steril. Uncovered disk (n = 16) yang
juga ditempatkan ke piring tes yang berisi angka setara spesies sasaran sebagai
kontrol terungkap tambahan (kontrol 2). Bobot logam kecil (12 g) dari nilon mesh
pads kemudian diterapkan pada permukaan atas setiap hidrogel persegi agar
kontak antara gel, selulosa disk dan permukaan agar-agar tidak terganggu dengan gel lanjut
pembengkakan (sekitar 20% lebih dari 12 jam) , berikut tahap ekuilibrium awal.
uji piring besar dengan disk dan gel diinkubasi pada 25 ° C selama durasi
percobaan.