However, there are no good laboratory tools with the capacity to confirm what occurs during the other months. Cross-reaction with some related flaviviruses, such as dengue 1,2,3, and 4, and with yellow fever and West Nile viruses is common, creating a circumstance where proper diagnosis of ZIKV may be difficult to achieve.
Then, a plaque-reduction neutralization testing can be performed to measure virus-specific neutralizing antibodies and differentiate between cross-reacting antibodies in primary flavivirus infections.Due to a routine cross-reaction that is common in patients with dengue virus, patients with ZIKV infection should be evaluated for possible dengue virus infection.