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A B S T R A C TFor the analysis of cell type-specific miRNA expression patterns qPCR is currently the method of choiceowing to its high accuracy. However, to obtain reliable results, a proper normalization strategy is anabsolute prerequisite, which is often underestimated. To demonstrate the importance of using a set ofsuitable reference genes, we tested two normalization strategies by comparing gene expressions oftissue-specific miRNA targets normalized against: (1) previously validated endogenous controls (miR92and miR374) and (2) a commonly used miRNA reference
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