Amplification was done in a TC-412 thermal cycler (Techne, Cambridge Ltd., UK) using the following programs:(1) for the 16S rRNA gene, initial denaturation for 5 minutes at 95 °C, followed by 35 cycles of 30 seconds at 95 °C, 30 seconds at 55 °C, and 1.5 minutes at 72 °C, and final elongation step of 5 minutes at 72 °C and (2) for the cpcBA-IGS region, the thermal cycling was performed with an initial denaturation at 94 °C for 5 minutes followed by 40 cycles of 94 °C for 20 seconds, 58 °C for 30 seconds,72 °C for 1 minute with a final elongation step of 72 °C for 5 minutes.