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The effect of combined treatment o fantimicrobial coatings and g-radiation on reduction of foodpathogens such as Listeria monocyto genes, Escherichia coli, and Salmonella Typhimurium was evaluatedin broccoli florets.Broccoli florets were inoculated with pathogenic bacteria at 106 CFU/g. Inoculatedflorets were then coated with methyl cellulose-based coating containing various mixtures of anti-microbial agents : organic acids(OAs) plus lactic acid bacteria metabolites(LABs),OA plus citrus extract(CE), OA plus CE plus spice mixture(SM),and OA plus rosemary extract(RE).Coated florets wereirradiated with various doses(0–3.3kGy),and microbial analyses were used to calculate the D10 valueand radio sensitive relative.The coating containing OA plus CE was the most effective for mulation forincreasing the sensitization of Escherichia coli by 2.4 times as compared to the control with out theantimicrobial coating.For Salmonella Typhimurium,coating containing OA plus LAB was the mosteffective for mulation , increasing radio sensitivity by 2.4 times as well.All antimicrobial coating shadalmost the same effect of increasing the sensitivity of Listeria monocyto genes (from 1.31to1.45times)to g-irradiation.
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