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this review,wefocusedontheuseofantimicrobialtestingmethods forthe in vitro investigationofextractsandpuredrugsaspotentialantimicrobialagents.After therevolutioninthe “golden era”, whenalmostallgroupsof importantantibiotics(tetracyclines,cephalosporins,aminogly-cosides andmacrolides)werediscoveredandthemainproblemsof chemotherapyweresolvedinthe1960s,thehistoryrepeatsitself nowadaysandtheseexcitingcompoundsareindangeroflosing theirefficacy becauseoftheincreaseinmicrobialresistance[1]. Currently,itsimpactisconsiderablewithtreatmentfailuresassociatedwithmultidrug-resistantbacteriaandithasbecomeaglobal concerntopublichealth [2,3]
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