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•Uses for determination of the MIC of antibiotics, antifungals dan antimycobacterials•MIC value is determined at the intersection of the strip and the growth inhibition ellipse•A good correlation between the MIC values determined by Etest and those obtained by broth dilution or agar dilution method•Can also be performed to investigate the antimicrobial interaction between two drugsan Etest strip impregnated with a first antibiotic, is placed on a pre-inoculated agar plate surface. After 1 hour, the strip removed and replaced by another impregnated with a secondn antibiotic. The synergy is detected by a decrease of the MIC of the combinatiob by at least two dilutions compared to that of the most active antibiotic testes aloneAntimicrobial gradient method (Etest)
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